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What kind of machines? Which operating systems? What exactly do these machines do? (Workstations, Servers, something else?)
You mention that "where all the machines in a particluar region go slow".. What type of "region" are you referring to? How much memory EXACTLY do you mean? (Check Task Manager/Processes tab?)
Have you got the newest 5300 scan engine installed?
Hope this helps and let us know more.