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You'll need at least patch 5 or patch 6 installed on your ePO 4.0 before upgrading to ePO 4.5 patch 1.
Once upgraded new agent will only be distributed if you have an active deployment task for it or if you have enabled global update.
Minimum requirements, and any 'gotcha's' to be aware of are also listed in the Readme.html file that ships with ePO 4.5 Patch 1 (and ePO 4.5 RTW).
Recommended reading before running your upgrade.