I'm trying to exclude all files in a directory, and all the files in that directory's subdirectories.
In ePO, would i just make an exclusion for
then check the also exclude subdirectories?
What you have listed would be correct. Also have a look at the following link for additional information on creating exclusions.
Managing file and folder exclusions in VirusScan Enterprise 8.x
Using system variables with VirusScan Enterprise 8.x