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The user profile service failed. User profile cannot be loaded

I have some users getting this prompt after some settings were made to the Access Protection Policy "The user profile service failed. User profile cannot be loaded"

Does anyone know which Access Protection Policy setting would cause this login issue? I have looked at the access protection logs and I am not seeing anything that screams "this is it". Also, some of the system were in the ePO 4.5 server's "Lost&Found" OU, but I don't believe that should have caused the issue.

I have set everything back to the default application default settings, but that is not going to be a solution going forward. Any assistance would be greatly appreciated. I have enclosed a screenshot of the settings before and after for more understanding.

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